7T97
Crystal structure of engineered CYS-CYS fab dimer CH1-207 (HC4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.377, 141.075, 118.605 |
| Unit cell angles | 90.00, 90.32, 90.00 |
Refinement procedure
| Resolution | 75.690 - 2.144 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (20-MAY-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.690 | 75.690 | 2.800 |
| High resolution limit [Å] | 2.144 | 8.390 | 2.650 |
| Rmerge | 0.111 | 0.033 | 0.607 |
| Rmeas | 0.131 | 0.039 | 0.714 |
| Rpim | 0.070 | 0.021 | 0.374 |
| Total number of observations | 8067 | 37405 | |
| Number of reflections | 90661 | 2331 | 10469 |
| <I/σ(I)> | 9.2 | 22 | 2.4 |
| Completeness [%] | 99.8 | 99.3 | 99.9 |
| Redundancy | 3.5 | 3.5 | 3.6 |
| CC(1/2) | 0.995 | 0.999 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8 M sodium succinate |
