7T8S
Light Harvesting complex phycoerythrin PE 566, from the cryptophyte Cryptomonas pyrenoidifera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.2632 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 178.359, 178.359, 173.546 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.770 - 2.000 |
R-factor | 0.2045 |
Rwork | 0.203 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lms |
RMSD bond length | 0.005 |
RMSD bond angle | 0.852 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (v0.7.4) |
Phasing software | PHASER (V2.8.3) |
Refinement software | PHENIX (1.20rc2_4400) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.770 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 1.529 |
Rmeas | 0.080 | |
Rpim | 0.056 | 0.930 |
Number of reflections | 187274 | 9197 |
<I/σ(I)> | 6.18 | 0.9 |
Completeness [%] | 99.8 | 100 |
Redundancy | 2 | 7.2 |
CC(1/2) | 0.995 | 0.572 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 20-25% PEG4000, 0.1 M HEPES |