7T8K
BrxR from Acinetobacter BREX type I phage restriction system bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.586, 71.477, 87.413 |
Unit cell angles | 90.00, 105.13, 90.00 |
Refinement procedure
Resolution | 64.220 - 2.300 |
R-factor | 0.1979 |
Rwork | 0.196 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | apo BrxR |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.960 | 2.300 |
Rmerge | 0.096 | 0.063 | 0.612 |
Rmeas | 0.105 | 0.068 | 0.668 |
Rpim | 0.042 | 0.027 | 0.264 |
Total number of observations | 239308 | ||
Number of reflections | 38735 | 3987 | 3929 |
<I/σ(I)> | 11.3 | ||
Completeness [%] | 97.5 | 97.6 | 100 |
Redundancy | 6.2 | 6.1 | 6.1 |
CC(1/2) | 0.997 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 300 | 0.04 M Citric acid, 20% w/v PEG 3350, 0.06 M BIS-TRIS propane, pH 6.4 |