7T7Z
The crystal structure of family 8 carbohydrate-binding module from Dictyostelium discoideum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.45863 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 36.739, 47.270, 79.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.620 - 1.460 |
R-factor | 0.154 |
Rwork | 0.152 |
R-free | 0.19090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7t7y |
Data reduction software | iMOSFLM (7.3.0) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.270 | 47.270 | 1.490 |
High resolution limit [Å] | 1.460 | 8.020 | 1.460 |
Rmerge | 0.072 | 0.062 | 0.229 |
Rmeas | 0.076 | 0.065 | 0.266 |
Rpim | 0.023 | 0.022 | 0.131 |
Total number of observations | 215097 | 1781 | 3985 |
Number of reflections | 23773 | 192 | 1049 |
<I/σ(I)> | 19.4 | 30.7 | 4.8 |
Completeness [%] | 96.9 | 99.8 | 90.9 |
Redundancy | 9 | 9.3 | 3.8 |
CC(1/2) | 0.998 | 0.996 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.6 M di-sodium DL-malate |