7T7U
Light Harvesting complex phycocyanin PC 630, from the cryptophyte Chroomonas sp. M1627
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95369 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 90.294, 93.411, 132.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.580 - 1.800 |
R-factor | 0.1593 |
Rwork | 0.158 |
R-free | 0.18490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lms |
RMSD bond length | 0.012 |
RMSD bond angle | 1.260 |
Data reduction software | MOSFLM (2.0.6) |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.500 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.096 | 0.047 |
Rmeas | 0.123 | 0.702 |
Rpim | 0.076 | 0.333 |
Number of reflections | 51263 | 7267 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 98.8 | |
Redundancy | 4.3 | |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | PEG 3,350 20-28%, HEPES 100 mM pH7.5 |