7T7N
Structure of SPCC1393.13 protein from fission yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-23 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.5895 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.720, 88.221, 48.257 |
Unit cell angles | 90.00, 101.07, 90.00 |
Refinement procedure
Resolution | 47.360 - 2.000 |
R-factor | 0.1621 |
Rwork | 0.160 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7t7k |
RMSD bond length | 0.008 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.360 | 2.050 |
High resolution limit [Å] | 1.990 | 1.990 |
Rpim | 0.048 | 0.311 |
Number of reflections | 31632 | 1004 |
<I/σ(I)> | 12.7 | 2.8 |
Completeness [%] | 90.5 | |
Redundancy | 6.1 | |
CC(1/2) | 0.995 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.24 mM SPCC1393.13 protein in a buffer containing 5 mM CoCl2, 10 mM sodium pyrophosphate (pH 6.5), 18 mM Tris-HCl (pH 7.5), and 135 mM NaCl mixed with precipitant solution containing 0.1 M citric acid (pH 5.0) and 20% (w/v) PEG-6000 |