7T7I
EBV nuclear egress complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.2822 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 238.153, 238.153, 137.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.520 - 3.970 |
R-factor | 0.2722 |
Rwork | 0.270 |
R-free | 0.30810 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.745 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 168.540 | 4.190 |
High resolution limit [Å] | 3.970 | 3.970 |
Rmerge | 0.285 | 3.213 |
Rmeas | 0.305 | 3.403 |
Rpim | 0.079 | 0.852 |
Number of reflections | 34632 | 4965 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 15 | 15.8 |
CC(1/2) | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20 mM HEPES pH 8.0, 100 mM NaCl, 0.5 mM TCEP in the hanging drop. 25% PEG 3350, 0.1 M Tris-HCl pH 8.5, 0.2 M Li2SO4 in the reservoir. |