7T63
Crystal structure of a delta 6 18:0-ACP desaturase from Thunbergia laurifolia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.74333 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.896, 129.700, 62.996 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.820 - 2.000 |
R-factor | 0.2085 |
Rwork | 0.208 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1afr |
RMSD bond angle | 17.216 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 90410 | 1389 |
<I/σ(I)> | 10.5 | |
Completeness [%] | 84.6 | |
Redundancy | 7.6 | |
CC(1/2) | 0.996 | 0.576 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.1M BICINE, 2-4% v/v 1,4-Dioxane, 10% w/v Polyethylene glycol 20000 |