7T5V
Structure of E. coli CapH C-terminal domain I99M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 38.383, 38.383, 59.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.240 - 1.260 |
R-factor | 0.1924 |
Rwork | 0.191 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ideal alpha helix |
RMSD bond length | 0.011 |
RMSD bond angle | 0.896 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.430 | 1.280 |
High resolution limit [Å] | 1.260 | 1.260 |
Rmerge | 0.039 | 0.732 |
Rpim | 0.017 | 0.460 |
Number of reflections | 12610 | 597 |
<I/σ(I)> | 21.1 | 2 |
Completeness [%] | 99.8 | 98.3 |
Redundancy | 5.9 | |
CC(1/2) | 0.999 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 25 mM MgCl2, 30% PEG 550 MME |