7T5H
Structure of rabies virus phosphoprotein C-terminal domain, wild type
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.312, 43.987, 61.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.830 - 1.500 |
R-factor | 0.1866 |
Rwork | 0.185 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vyi |
RMSD bond length | 0.007 |
RMSD bond angle | 0.873 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.990 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.085 | 1.463 |
Rmeas | 0.088 | 1.544 |
Rpim | 0.025 | 0.411 |
Number of reflections | 18982 | 928 |
<I/σ(I)> | 14.9 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 12.7 | |
CC(1/2) | 0.999 | 0.816 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.75 | 281 | 1.9 M ammonium sulfate, 0.2 M potassium sodium tartrate and 0.1 M sodium citrate (pH 4.75) |