7T5H

Structure of rabies virus phosphoprotein C-terminal domain, wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	42.312, 43.987, 61.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.830 - 1.500
R-factor	0.1866
Rwork	0.185
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyi
RMSD bond length	0.007
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.990	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.085	1.463
Rmeas	0.088	1.544
Rpim	0.025	0.411
Number of reflections	18982	928
<I/σ(I)>	14.9	1.6
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.75	281	1.9 M ammonium sulfate, 0.2 M potassium sodium tartrate and 0.1 M sodium citrate (pH 4.75)