7T58
Crystal structure of Miz1 BTB domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-22 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.978 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.919, 115.898, 64.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.305 - 2.053 |
R-factor | 0.184587497565 |
Rwork | 0.182 |
R-free | 0.23149 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q81 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.425 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.305 | 2.127 |
High resolution limit [Å] | 2.053 | 2.053 |
Rmerge | 0.117 | 0.438 |
Number of reflections | 13322 | 1025 |
<I/σ(I)> | 11.03 | |
Completeness [%] | 91.4 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% w/v PEG1500 |