7T4Z
Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in ligand-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.954 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 106.492, 128.908, 67.687 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.680 - 1.780 |
R-factor | 0.1884 |
Rwork | 0.187 |
R-free | 0.21090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1atg |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.680 | 1.820 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.067 | 0.518 |
Rmeas | 0.073 | 0.562 |
Rpim | 0.028 | 0.214 |
Number of reflections | 44640 | 2473 |
<I/σ(I)> | 16.7 | 3.5 |
Completeness [%] | 99.9 | 98.2 |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293.15 | 0.1 M sodium citrate pH 3.5, 2.0 M ammonium sulfate, 25% v/v glycerol |