7T4U
Crystal Structure of cGMP-dependent Protein Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.463, 102.709, 103.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.360 - 1.990 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.024 | 2.169 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.170 | 1.278 |
| Rpim | 0.075 | 0.557 |
| Number of reflections | 45643 | 2283 |
| <I/σ(I)> | 8.8 | 1.5 |
| Completeness [%] | 67.6 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.1M MIB pH 9.0 25% PEG 1500 |
