7T4D
Pore structure of pore-forming toxin Epx4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 122.301, 122.301, 128.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.210 - 3.000 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ygt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.663 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.210 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.204 | 0.927 |
| Number of reflections | 18919 | 1855 |
| <I/σ(I)> | 8.94 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277.15 | 5% (w/v) polyethylene glycol 8000, 40% (v/v) MPD, 0.1 M Sodium Cacodylate, pH 6.5 |
