7T34
Crystal structure of AtDHDPR1 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 118.840, 118.840, 127.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.050 - 2.890 |
R-factor | 0.1908 |
Rwork | 0.188 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1arz |
RMSD bond length | 0.009 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.050 | 2.993 |
High resolution limit [Å] | 2.889 | 2.889 |
Rmerge | 0.044 | 0.120 |
Rmeas | 0.046 | 0.124 |
Number of reflections | 11082 | 11082 |
<I/σ(I)> | 30.28 | 1.79 |
Completeness [%] | 99.7 | 97.74 |
Redundancy | 13.09 | |
CC(1/2) | 1.000 | 0.846 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1 M bis-tris hydrochloride (pH 6.5), 0.245 M magnesium formate, 22% (w/v) PEG3350 |