7T28
Structure of phage Bsp38 anti-Pycsar nuclease Apyc1 in apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-10-19 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 82.485, 82.485, 76.098 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.260 - 2.680 |
R-factor | 0.3021 |
Rwork | 0.300 |
R-free | 0.31970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alphafold2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.484 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.420 | 2.830 |
High resolution limit [Å] | 2.680 | 2.700 |
Rpim | 0.071 | 0.732 |
Number of reflections | 8581 | 1129 |
<I/σ(I)> | 5.9 | 1.1 |
Completeness [%] | 100.0 | |
Redundancy | 13.3 | |
CC(1/2) | 0.996 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M lithium sulfate, 0.1 M Tris-HCl pH 7.5, 30% PEG-4000 |