7T26
Structure of phage FBB1 anti-CBASS nuclease Acb1 in apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-09-24 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 43.845, 43.845, 154.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.170 - 1.140 |
R-factor | 0.161 |
Rwork | 0.160 |
R-free | 0.18080 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.280 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.190 | 1.170 |
High resolution limit [Å] | 1.140 | 1.150 |
Rpim | 0.012 | 0.377 |
Number of reflections | 53419 | 2479 |
<I/σ(I)> | 23.3 | 1.4 |
Completeness [%] | 99.5 | 95.3 |
Redundancy | 24 | 12.7 |
CC(1/2) | 1.000 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 M Ammonium Sulfate, 0.1 M Sodium Citrate pH 4.6 |