7T1V
Crystal structure of an equine H7 hemagglutinin from A/equine/NY/49/73 (H7N7) in complex with 3'-GcLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 3 |
| Unit cell lengths | 112.753, 112.753, 130.187 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.050 |
| R-factor | 0.2183 |
| Rwork | 0.217 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n5a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.428 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmeas | 0.101 | |
| Rpim | 0.032 | 0.340 |
| Number of reflections | 116123 | 5442 |
| <I/σ(I)> | 16.1 | 1 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.4 | |
| CC(1/2) | 0.990 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 293 | 32% (w/v) polyethylene glycol 400 and 0.1 M CAPS, pH 10 |
