7T19

Rev1 Ternary Complex with dGTP and Ca2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-13
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	62.183, 73.599, 117.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.590 - 2.010
R-factor	0.1864
Rwork	0.181
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6x6z
RMSD bond length	0.009
RMSD bond angle	1.053
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.060
High resolution limit [Å]	2.010	2.030
Number of reflections	36239	1772
<I/σ(I)>	6.87
Completeness [%]	99.5
Redundancy	4.4
CC(1/2)	0.993	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15-23% PEG3350, 200 mM ammonium nitrate