7T0D
Cryptococcus neoformans protein farnesyltransferase in complex with FPP and inhibitor 2k
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.915, 141.915, 130.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.430 - 1.906 |
| R-factor | 0.1738 |
| Rwork | 0.173 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sfx |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.430 | 1.974 |
| High resolution limit [Å] | 1.906 | 1.906 |
| Rmerge | 0.063 | 0.720 |
| Rmeas | 0.069 | 0.788 |
| Rpim | 0.028 | 0.316 |
| Number of reflections | 103127 | 9878 |
| <I/σ(I)> | 15.61 | 2.59 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 6 | 6 |
| CC(1/2) | 0.998 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 290 | 100mM CAPSO pH9.5, 50-75mM Li2SO4, 200mM NaCl, 16%-21% PEG4000. |
