7SZY

High-resolution structure of the nuclease domain from the main replication protein NS1 of Human Parvovirus B19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 2 21 21
Unit cell lengths	45.060, 49.100, 74.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.060 - 2.400
R-factor	0.2009
Rwork	0.198
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6usm
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.060	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.102	0.533
Rmeas	0.145	0.754
Rpim	0.102	0.533
Number of reflections	6799	665
<I/σ(I)>	7.21	2.43
Completeness [%]	98.4	98.66
Redundancy	2	2
CC(1/2)	0.981	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	290	15% PEG 3350 0.1M sodium citrate pH 4.5 0.1M NaCl 0.1M LiCl