7SZT
Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters in low pH (protonated state)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-09-01 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 |
Unit cell lengths | 49.704, 74.323, 107.433 |
Unit cell angles | 93.56, 89.71, 109.92 |
Refinement procedure
Resolution | 32.680 - 2.320 |
R-factor | 0.2537 |
Rwork | 0.251 |
R-free | 0.29450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wk8 |
Data reduction software | DIALS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.210 | 2.670 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.089 | 0.400 |
Rmeas | 0.104 | |
Number of reflections | 26109 | 1305 |
<I/σ(I)> | 6.5 | |
Completeness [%] | 80.3 | 45.6 |
Redundancy | 3.8 | |
CC(1/2) | 0.990 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40% PEG600, sodium acetate, pH 5.0, 100 mM calciium acetate, Apo12 |