7SZG
Structure of the Rieske Non-heme Iron Oxygenase GxtA Pressurized with Xenon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 2.06633 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.730, 96.932, 80.809 |
Unit cell angles | 90.00, 106.97, 90.00 |
Refinement procedure
Resolution | 48.470 - 2.690 |
R-factor | 0.2067 |
Rwork | 0.205 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wnc |
RMSD bond length | 0.011 |
RMSD bond angle | 1.579 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmeas | 0.141 | 1.393 |
Number of reflections | 59952 | 4447 |
<I/σ(I)> | 19.63 | 2.42 |
Completeness [%] | 99.7 | 100 |
Redundancy | 18.5 | 17.45 |
CC(1/2) | 0.999 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 0.3 M MgCl2, 0.1 M Bis-Tris pH 5.5, 25% v/v PEG3350, 15% v/v glycerol |