7SZ9
Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabB, and C16:1-crypto Acyl Carrier Protein, AcpP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.450, 103.580, 79.280 |
Unit cell angles | 90.00, 108.28, 90.00 |
Refinement procedure
Resolution | 54.020 - 2.200 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6okc |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 75.280 | 60.900 | 2.240 |
High resolution limit [Å] | 2.200 | 11.220 | 2.200 |
Rmerge | 0.205 | ||
Rmeas | 0.238 | ||
Rpim | 0.117 | ||
Number of reflections | 38093 | 372 | 1412 |
<I/σ(I)> | 3.7 | ||
Completeness [%] | 82.1 | 98.3 | 53.9 |
Redundancy | 2.1 | ||
CC(1/2) | 0.945 | 0.986 | 0.576 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 281 | 20% PEG 8000, 0.3 M sodium acetate, 0.1 M sodium cacodylate |