7SZ3

Mouse PARP13/ZAP ZnF5-WWE1-WWE2 bound to ADPr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-09-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.18057
Spacegroup name	P 31 2 1
Unit cell lengths	87.415, 87.415, 128.741
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.1959
Rwork	0.193
R-free	0.23050
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.528
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.085	2.270
Rmeas	0.087	2.321
Rpim	0.018	0.483
Total number of observations	648133
Number of reflections	29530	2519
<I/σ(I)>	28.7
Completeness [%]	100.0	99.9
Redundancy	21.9	22.4
CC(1/2)	1.000	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	1.4 M Na/K phosphate pH 5.5