7SZ2

Mouse PARP13/ZAP ZnF5-WWE1-WWE2 bound to ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-09-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.18057
Spacegroup name	P 31 2 1
Unit cell lengths	87.541, 87.541, 129.829
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.2058
Rwork	0.203
R-free	0.23840
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.437
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.770	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.062	1.639
Rmeas	0.063	1.677
Rpim	0.014	0.352
Total number of observations	652181
Number of reflections	29855	2545
<I/σ(I)>	33.8
Completeness [%]	100.0	100
Redundancy	21.8	22.5
CC(1/2)	1.000	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	1.4 M Na/K phosphate pH 5.5