7SYZ
Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 165.757, 165.757, 202.066 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.250 - 2.860 |
R-factor | 0.2464 |
Rwork | 0.246 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d11 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 6.140 | 2.850 |
Rmerge | 0.148 | 0.095 | 1.208 |
Total number of observations | 519266 | ||
Number of reflections | 38338 | 4146 | 3543 |
<I/σ(I)> | 9.7 | ||
Completeness [%] | 99.4 | 99.6 | 94.2 |
Redundancy | 13.5 | 12.8 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4% v/v Polyethylene glycol 400, 0.1 M Imidazole pH 7.0, 23% w/v Polyethylene glycol monomethyl ether 5,000 |