7SXP

G-quadruplex structure formed in the NRAS mRNA with a G8U substitution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.104
Spacegroup name	P 31 2 1
Unit cell lengths	36.402, 36.402, 70.103
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.530 - 2.900
R-factor	0.2421
Rwork	0.241
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.530	2.420
High resolution limit [Å]	2.300	2.320
Rpim	0.058	0.678
Number of reflections	2556	305
<I/σ(I)>	5.3	1.2
Completeness [%]	99.6
Redundancy	17.4
CC(1/2)	0.996	0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	20 - 25 % PEG 3350, 80 mM sodium chloride and 100 mM BIS-TRIS pH 6.5