7SWT
Crystal structure of the chromoprotein eforRED
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-04 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.95372 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.348, 75.859, 175.791 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.890 - 2.005 |
R-factor | 0.2111 |
Rwork | 0.209 |
R-free | 0.25020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7swr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.896 | 2.222 |
High resolution limit [Å] | 2.005 | 2.005 |
Rmerge | 0.186 | 1.300 |
Rmeas | 0.202 | 1.355 |
Rpim | 0.053 | 0.374 |
Number of reflections | 34510 | 1725 |
<I/σ(I)> | 12.4 | 1.9 |
Completeness [%] | 87.5 | 87.8 |
Redundancy | 13.4 | |
CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% (w/v) polyethylene glycol 8000, 0.2 M sodium acetate, 0.1 M sodium MES (pH 6) |