7SWS
Crystal structure of the chromoprotein amilCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.524, 131.742, 93.671 |
| Unit cell angles | 90.00, 100.75, 90.00 |
Refinement procedure
| Resolution | 92.030 - 1.642 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7swr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.026 | 1.797 |
| High resolution limit [Å] | 1.642 | 1.642 |
| Rmerge | 0.112 | 1.181 |
| Rmeas | 0.121 | 1.271 |
| Rpim | 0.045 | 0.467 |
| Number of reflections | 108877 | 5444 |
| <I/σ(I)> | 11.6 | 1.7 |
| Completeness [%] | 87.2 | 98 |
| Redundancy | 7.1 | 7.3 |
| CC(1/2) | 0.999 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% w/v polyethylene glycol 3350, 0.2 M sodium bromide, 0.1 M bis-tris propane chloride (pH 6.5) |
