7SWR
Crystal structure of the chromoprotein gfasPurple
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95374 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 92.136, 92.136, 78.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.930 - 1.388 |
R-factor | 0.1627 |
Rwork | 0.162 |
R-free | 0.17260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ir8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.136 | 1.467 |
High resolution limit [Å] | 1.388 | 1.388 |
Rmerge | 0.125 | 2.210 |
Rmeas | 0.126 | 2.249 |
Rpim | 0.026 | 0.582 |
Number of reflections | 56552 | 2826 |
<I/σ(I)> | 21.6 | 1.7 |
Completeness [%] | 96.1 | 71.7 |
Redundancy | 46.4 | 26.2 |
CC(1/2) | 1.000 | 0.708 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v polyethylene glycol 3350, 0.2 M Potassium thiocyanate |