7SWR
Crystal structure of the chromoprotein gfasPurple
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 92.136, 92.136, 78.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 1.388 |
| R-factor | 0.1627 |
| Rwork | 0.162 |
| R-free | 0.17260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ir8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.136 | 1.467 |
| High resolution limit [Å] | 1.388 | 1.388 |
| Rmerge | 0.125 | 2.210 |
| Rmeas | 0.126 | 2.249 |
| Rpim | 0.026 | 0.582 |
| Number of reflections | 56552 | 2826 |
| <I/σ(I)> | 21.6 | 1.7 |
| Completeness [%] | 96.1 | 71.7 |
| Redundancy | 46.4 | 26.2 |
| CC(1/2) | 1.000 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v polyethylene glycol 3350, 0.2 M Potassium thiocyanate |
