7SWC

MicroED structure of proteinase K from a 550 nm thick lamella measured at 300 kV

Experimental procedure

Collection date	2020-10-01
Spacegroup name	P 43 21 2
Unit cell lengths	68.510, 68.510, 104.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.410 - 2.900
R-factor	0.241
Rwork	0.239
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.004
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.436	0.926
Rmeas	0.534	1.133
Rpim	0.231
Number of reflections	27529	2085
<I/σ(I)>	2.43	1.14
Completeness [%]	90.0	90.4
Redundancy	2.8
CC(1/2)	0.773	0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1