7SWB

MicroED structure of proteinase K from a 360 nm thick lamella measured at 300 kV

Experimental procedure

Collection date	2020-10-01
Spacegroup name	P 43 21 2
Unit cell lengths	68.550, 68.550, 103.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.820 - 2.050
R-factor	0.1944
Rwork	0.191
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.012
RMSD bond angle	1.137
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.228	0.766
Rmeas	0.286	0.961
Rpim	0.128
Number of reflections	76827	5793
<I/σ(I)>	3.8	1.57
Completeness [%]	92.9	93.8
Redundancy	2.8
CC(1/2)	0.956	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1