7SW7

MicroED structure of proteinase K from a 530 nm thick lamella measured at 200 kV

Experimental procedure

Collection date	2020-11-14
Spacegroup name	P 43 21 2
Unit cell lengths	67.550, 67.550, 103.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.250 - 2.300
R-factor	0.2036
Rwork	0.202
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.390
High resolution limit [Å]	2.300	2.300
Rmerge	0.397
Rmeas	0.446	1.397
Rpim	0.197
Number of reflections	52756	5680
<I/σ(I)>	4.41	1.3
Completeness [%]	90.0	91.8
Redundancy	5.3
CC(1/2)	0.917	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1