7SW5
MicroED structure of proteinase K from a 460 nm thick lamella measured at 200 kV
Experimental procedure
| Collection date | 2020-11-14 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.000, 68.000, 102.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 1.950 |
| R-factor | 0.2029 |
| Rwork | 0.201 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cl7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.320 | |
| Rmeas | 0.357 | 1.644 |
| Rpim | 0.154 | |
| Number of reflections | 88464 | 6261 |
| <I/σ(I)> | 3.85 | 0.9 |
| Completeness [%] | 91.9 | 94 |
| Redundancy | 5.3 | |
| CC(1/2) | 0.963 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
