7SW4
MicroED structure of proteinase K from a 540 nm thick lamella measured at 200 kV
Experimental procedure
Collection date | 2020-11-14 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.720, 67.720, 102.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.160 - 2.400 |
R-factor | 0.1945 |
Rwork | 0.193 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cl7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.872 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.450 |
Rmerge | 0.325 | |
Rmeas | 0.386 | 1.057 |
Rpim | 0.156 | |
Number of reflections | 47088 | 4978 |
<I/σ(I)> | 3.26 | 1.9 |
Completeness [%] | 78.6 | 79.4 |
Redundancy | 3.3 | |
CC(1/2) | 0.925 | 0.486 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |