7SW1
MicroED structure of proteinase K from a 115 nm thick lamella measured at 200 kV
Experimental procedure
| Collection date | 2020-11-14 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.810, 67.810, 102.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.550 - 1.850 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cl7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.240 | |
| Rmeas | 0.265 | 1.590 |
| Rpim | 0.108 | |
| Number of reflections | 101309 | 6448 |
| <I/σ(I)> | 5.2 | 0.9 |
| Completeness [%] | 86.0 | 88.5 |
| Redundancy | 5.6 | |
| CC(1/2) | 0.988 | 0.306 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
