7SW0
MicroED structure of proteinase K from a 325 nm thick lamella measured at 120 kV
Experimental procedure
Collection date | 2020-12-11 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.781, 66.781, 99.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.430 - 2.700 |
R-factor | 0.2442 |
Rwork | 0.241 |
R-free | 0.28520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cl7 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.656 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.860 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.640 | 1.765 |
Rmeas | 0.708 | 1.951 |
Rpim | 0.296 | |
Number of reflections | 30326 | 4720 |
<I/σ(I)> | 3.2 | 1.02 |
Completeness [%] | 82.6 | 83 |
Redundancy | 5.5 | |
CC(1/2) | 0.893 | 0.359 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |