7SVZ
MicroED structure of proteinase K from a 200 nm thick lamella measured at 120 kV
Experimental procedure
| Collection date | 2020-12-11 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.971, 65.971, 102.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.340 - 2.000 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cl7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.852 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.279 | 1.516 |
| Rmeas | 0.309 | 1.667 |
| Rpim | 0.128 | |
| Number of reflections | 75287 | 11811 |
| <I/σ(I)> | 4.9 | 1.07 |
| Completeness [%] | 87.7 | 88.1 |
| Redundancy | 5.4 | |
| CC(1/2) | 0.983 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
