7SVY
MicroED structure of proteinase K from a 130 nm thick lamella measured at 120 kV
Experimental procedure
Collection date | 2020-12-11 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.838, 66.838, 100.502 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.080 - 2.300 |
R-factor | 0.2241 |
Rwork | 0.222 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cl7 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.789 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.327 | 1.551 |
Rmeas | 0.361 | 1.704 |
Rpim | 0.149 | |
Number of reflections | 49740 | 7763 |
<I/σ(I)> | 5 | 0.71 |
Completeness [%] | 82.5 | 84 |
Redundancy | 5.6 | |
CC(1/2) | 0.975 | 0.354 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |