7SVI
Bile Salt Hydrolase C from Lactobacillus johnsonii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 58.066, 80.882, 324.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.290 - 1.450 |
R-factor | 0.1833 |
Rwork | 0.183 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hez |
RMSD bond length | 0.005 |
RMSD bond angle | 0.792 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.290 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.049 | 0.680 |
Rmeas | 0.070 | 0.961 |
Rpim | 0.050 | 0.680 |
Number of reflections | 263785 | 22181 |
<I/σ(I)> | 6.2 | 0.6 |
Completeness [%] | 97.3 | 82.8 |
Redundancy | 1.9 | 1.7 |
CC(1/2) | 0.994 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium Citrate:HCl, pH 5.6, 10% (w/v) PEG 4000, 10% (v/v) isopropanol. Crystals formed in a 2:1 protein (10.3 mg/mL) to mother liquor ratio. |