7SVH

Bile Salt Hydrolase B from Lactobacillus gasseri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	87.350, 167.376, 88.153
Unit cell angles	90.00, 98.98, 90.00

Refinement procedure

Resolution	29.020 - 1.560
R-factor	0.221
Rwork	0.221
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hez
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	1.616
High resolution limit [Å]	1.560	1.560
Rmerge	0.059	1.945
Rmeas	0.084	2.750
Rpim	0.059	1.945
Number of reflections	348378	33984
<I/σ(I)>	6.4	1.9
Completeness [%]	97.1	85
Redundancy	2	1.9
CC(1/2)	0.999	0.194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M MgCl2, 0.1 M Sodium Cacodyolate:HCl, pH 6.5, 20% (w/v) PEG 1000. Crystals formed in a 1:2 ratio of protein (10.75 mg/mL) to mother liquor.