7SVE
Bile Salt Hydrolase A from Lactobacillus acidophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.561, 92.953, 87.057 |
Unit cell angles | 90.00, 107.22, 90.00 |
Refinement procedure
Resolution | 46.480 - 2.150 |
R-factor | 0.1527 |
Rwork | 0.151 |
R-free | 0.20510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hez |
RMSD bond length | 0.007 |
RMSD bond angle | 0.885 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.480 | 2.220 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.055 | 0.323 |
Rmeas | 0.078 | 0.457 |
Rpim | 0.055 | 0.323 |
Number of reflections | 68775 | 6798 |
<I/σ(I)> | 10.5 | 2.7 |
Completeness [%] | 98.1 | 97.4 |
Redundancy | 1.8 | 1.8 |
CC(1/2) | 0.996 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.15 M DL-Malic Acid, pH 7.0, 20% (w/v) PEG 3350. Crystals formed in a 2:1 protein (11.6 mg/mL):mother liquor ratio. |