7SV0
NADPH-dependent cytochrome P450 reductase 2b from Sorghum bicolor (SbCPR2b) -oxidized form of NADP+ complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.947, 69.158, 150.949 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.830 - 2.700 |
R-factor | 0.2249 |
Rwork | 0.221 |
R-free | 0.26450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gxu |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.830 | 2.797 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.123 | 1.537 |
Number of reflections | 14106 | 1372 |
<I/σ(I)> | 10.18 | |
Completeness [%] | 99.8 | |
Redundancy | 5.5 | |
CC(1/2) | 0.994 | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M sodium thiocyanate, pH 6.9, 20% w/v PEG3350 |