7SUT

Light harvesting phycobiliprotein HaPE645 from the cryptophyte Hemiselmis andersenii CCMP644

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	54.032, 80.983, 115.837
Unit cell angles	90.00, 92.21, 90.00

Refinement procedure

Resolution	41.430 - 1.490
R-factor	0.1459
Rwork	0.145
R-free	0.17470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lmx
RMSD bond length	0.011
RMSD bond angle	1.317
Data reduction software	DIALS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX (1.14)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.750	1.520
High resolution limit [Å]	1.490	1.490
Rmerge	0.056	1.117
Number of reflections	151716	4645
<I/σ(I)>	9.9	0.9
Completeness [%]	93.4	58.3
Redundancy	3.6	2.2
CC(1/2)	0.998	0.463

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	300	0.1 M Bis-Tris + 25 % (w/v) PEG 3350