7SUO

Crystal Structure of the G3BP1 NTF2-like domain bound to the IDR1 of SARS-CoV-2 nucleocapsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	42.704, 51.140, 153.088
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.510 - 2.350
R-factor	0.2017
Rwork	0.197
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fw5
RMSD bond length	0.004
RMSD bond angle	0.699
Data reduction software	HKL-3000 (v721.3)
Data scaling software	HKL-3000 (v721.3)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.150	0.065	0.659
Rmeas	0.160	0.069	0.720
Rpim	0.056	0.025	0.281
Total number of observations	114088
Number of reflections	14524	1616	1318
<I/σ(I)>	4.6
Completeness [%]	98.8	100	92.4
Redundancy	7.9	7.5	6.1
CC(1/2)		0.998	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277.15	20% propan-2-ol, 0.1 M MES monohydrate (pH 6.0), 20% PEG MME 20,000