7SUL
Crystal structure of the WD-repeat domain of human SEC31A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.180, 156.750, 85.190 |
| Unit cell angles | 90.00, 99.48, 90.00 |
Refinement procedure
| Resolution | 44.370 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ALPHAFOLD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.116 | 0.052 | 0.781 |
| Rmeas | 0.136 | 0.061 | 0.917 |
| Rpim | 0.069 | 0.031 | 0.473 |
| Total number of observations | 167966 | ||
| Number of reflections | 49159 | 2555 | 1994 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 94.2 | 95.9 | 75 |
| Redundancy | 3.4 | 3.6 | 3 |
| CC(1/2) | 0.996 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 30%(w/v)PEG4K,0.2M Sodium Acetat, 0.1M Tris HCL pH8.5 |
