7SUB

3-oxoacyl-ACP reductase FabG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97922
Spacegroup name	P 31 2 1
Unit cell lengths	56.006, 56.006, 133.129
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.200 - 1.780
R-factor	0.187
Rwork	0.185
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hq1 2uvd 4rzh 4jro
RMSD bond length	0.015
RMSD bond angle	1.242
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.036	0.787
Rmeas	0.040	0.886
Rpim	0.017	0.401
Number of reflections	24038	1338
<I/σ(I)>	31.1	2.5
Completeness [%]	99.9	98.5
Redundancy	9.7	8.9
CC(1/2)	1.000	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M Sodium Malonate pH = 4.0 12% PEG 3350