7SU2

Crystal structure of a Co-bound RIDC1 variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.953690
Spacegroup name	P 1 21 1
Unit cell lengths	47.875, 90.024, 52.042
Unit cell angles	90.00, 95.91, 90.00

Refinement procedure

Resolution	37.000 - 2.000
R-factor	0.1871
Rwork	0.184
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bc5
RMSD bond length	0.013
RMSD bond angle	1.823
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.069
High resolution limit [Å]	2.000	2.000
Rmerge	0.017	0.059
Rmeas	0.024	0.084
Rpim	0.017	0.059
Number of reflections	58467	5607
<I/σ(I)>	26.68	7.4
Completeness [%]	98.4	96.01
Redundancy	2
CC(1/2)	1.000	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG400, 200 mM NaCl, 100 mM HEPES (7.5)