7STR
Crystal Structure of Human Fab S24-1063 in the Complex with the N-teminal Domain of Nucleocapsid Protein from SARS CoV-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-05 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.021, 67.134, 84.459 |
Unit cell angles | 90.00, 107.02, 90.00 |
Refinement procedure
Resolution | 40.940 - 1.500 |
R-factor | 0.1634 |
Rwork | 0.161 |
R-free | 0.20180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | swiss model |
RMSD bond length | 0.009 |
RMSD bond angle | 0.977 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.076 | 0.485 |
Number of reflections | 89494 | 3771 |
<I/σ(I)> | 24.6 | 1.34 |
Completeness [%] | 97.4 | 82.3 |
Redundancy | 4 | 2.6 |
CC(1/2) | 0.993 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis Tris HCl pH 5.5, 25 % (w/v) PEG 3350 |